Molecular cloning and characterization of Pif gene from pearl mussel, Hyriopsis cumingii, and the gene expression analysis during pearl formation
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چکیده
منابع مشابه
Cloning and Analysis of Gene Expression of Two Toll Receptors in Freshwater Pearl Mussel Hyriopsis cumingii
Toll receptors are involved in innate immunity of invertebrates. In this study, we identify and characterize two Toll genes (named HcToll4 and HcToll5) from triangle sail mussel Hyriopsis cumingii. HcToll4 has complete cDNA sequence of 3,162 bp and encodes a protein of 909 amino acids. HcToll5 cDNA is 4,501 bp in length and encodes a protein of 924 amino acids. Both deduced HcToll4 and HcToll5 ...
متن کاملMolecular characterization and expression pattern of an α-amylase gene (HcAmy) from the freshwater pearl mussel, Hyriopsis cumingii.
The freshwater pearl mussel Hyriopsis cumingii is of commercial importance because it produces the freshwater pearl; however, knowledge about the molecular characterization and regulation mechanisms of α-amylase remains unknown for this species. In this study, the full-length cDNA of the α-amylase gene (HcAmy) was isolated from H. cumingii by the rapid amplification of cDNA ends. Tissue-specifi...
متن کاملTranscriptome analysis of the freshwater pearl mussel, Hyriopsis cumingii (Lea) using illumina paired-end sequencing to identify genes and markers
The transcriptome of triangle sail mussel Hyriopsis cumingii (Lea) using Illumina paired-end sequencing technology was conducted and analyzed. Equal quantities of total RNA isolated from six tissues, including gonad, hepatopancreas, foot, mantel, gill and adductor muscle, were pooled to construct a cDNA library. A total of 58.09 million clean reads with 98.48 % Q20 bases were generated. Cluster...
متن کاملMolecular characterization and expression patterns of the big defensin gene in freshwater mussel (Hyriopsis cumingii).
Antimicrobial peptides (AMPs), of which big defensins are examples, are an important component of the natural defenses of most living organisms, and possess remarkable microbicidal activities. In the present study, using expressed-sequence tag sequences from a cDNA library and RACE, the full-length cDNA sequence of the big defensin gene from the triangle-shell pearl mussel, Hyriopsis cumingii, ...
متن کاملTranscriptome analysis of the freshwater pearl mussel, Hyriopsis cumingii (Lea) using illumina paired-end sequencing to identify genes and markers
The transcriptome of triangle sail mussel Hyriopsis cumingii (Lea) using Illumina paired-end sequencing technology was conducted and analyzed. Equal quantities of total RNA isolated from six tissues, including gonad, hepatopancreas, foot, mantel, gill and adductor muscle, were pooled to construct a cDNA library. A total of 58.09 million clean reads with 98.48 % Q20 bases were generated. Cluster...
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ژورنال
عنوان ژورنال: 3 Biotech
سال: 2018
ISSN: 2190-572X,2190-5738
DOI: 10.1007/s13205-018-1233-z